LOCUS       Exported                2056 bp DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2025 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
COMMENT     pSmart-HCAmp v3.2
FEATURES             Location/Qualifiers
     source          1..2056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    113..132
                     /label=Upstream Flanking Bases, TC added before insert
     misc_feature    133..196
                     /label=replaced by insert
     misc_feature    197..216
                     /label=Downstream Flanking Bases, G added behind insert
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      328..359
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14
                     transcriptionterminator"
     promoter        360..462
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene
                     encodingchloramphenicol acetyltransferase"
     CDS             463..1323
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1395..1422
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1434..2021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcgg ccgccggagg gttgcgtttg agacgggcga cagatatcag ttctggacga
      181 gcgagctgtc gtccgacgcg tgatcttacg gcattatacg tatgatcggt ccacgatcag
      241 ctagattatc tagtcagctt gatgtcatag ctgtttcctg aggctcaata ctgaccattt
      301 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt
      361 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      421 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagtat tcaacatttc
      481 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      541 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      601 ctggatctca acagcggtaa gatccttgag agtttacgcc ccgaagaacg ttttccaatg
      661 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      721 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      781 acagaaaagc atctcacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      841 atgagtgata acactgcggc caacttactt ctggcaacga tcggaggacc gaaggagcta
      901 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      961 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1021 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1081 gactggatgg aggcggataa agttgcagga tcacttctgc gctcggccct cccggctggc
     1141 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1201 ctggggccag atggtaagcc ctcccgcatc gtagttatct acacgacggg gagtcaggca
     1261 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1321 taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc ttgaggacct
     1381 aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat
     1441 aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac
     1501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa
     1921 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1981 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct
     2041 accgaagaaa ggccca
//
