LOCUS       Exported                1962 bp DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2025 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
COMMENT     pSmart-HCKan v3.2
FEATURES             Location/Qualifiers
     source          1..1962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    113..132
                     /label=Upstream Flanking Bases, TC added before insert
     misc_feature    133..195
                     /label=replaced by insert
                     /label=Digested in linearized plasmid
     misc_feature    196..215
                     /label=Downstream Flanking Bases, G added behind insert
     primer_bind     complement(263..279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      327..358
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        359..461
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             462..1277
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1301..1328
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1340..1927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcgg ccgccggagg gttgcgtttg agacgggcga cagatatcac tactggaccg
      181 cgagctgtgc tgcgacgcgt gatcttacgg cattatacgt atgatcggtc cacgatcagc
      241 tagattatct agtcagcttg atgtcatagc tgtttcctga ggctcaatac tgaccattta
      301 aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg
      361 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      421 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg
      481 aaacgtcttg cttgaagccg cgattaaatt ccaacatgga tgctgattta tatgggtata
      541 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
      601 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
      661 atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
      721 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat
      781 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
      841 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat
      901 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg
      961 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaactcttgc
     1021 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     1081 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     1141 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     1201 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc
     1261 tcgatgagtt tttctaatga ggacctaaat gtaatcacct ggctcacctt cgggtgggcc
     1321 tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1381 gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1741 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1801 ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1921 atctcaagaa gatcctttga ttttctaccg aagaaaggcc ca
//
