LOCUS       pUCIDT_Ampicilli        2752 bp DNA     circular SYN 03-OCT-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     pUC57-Amp v3.3 Blunt
FEATURES             Location/Qualifiers
     source          1..2752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    480..485
                     /label=Insertion Site - EcoRV
     primer_bind     complement(531..547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    555..571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(579..609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    624..645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(933..1521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1692..2552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2553..2657)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atatcagttc tggaccagcg agctgtgctg cgactcgtgg cgtaatcatg gtcatagctg
      541 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      601 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      661 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      721 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gtcgcttacc
     1081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1801 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2281 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2341 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2401 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2461 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2521 ataagggcga cacggaaatg ttgaatactc atactctacc tttttcaata ttattgaagc
     2581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2701 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//