LOCUS       pUCIDT_Kanamycin        2705 bp DNA     circular SYN 03-OCT-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   cgillmore
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    480..485
                     /label=Insertion site - EcoRV
     primer_bind     complement(531..547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    555..571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(579..609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    624..645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(933..1521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1692..2507)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRFAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(2508..2610)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg agaacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttaaga cgggcgacag
      481 atatcagttc tggaccagcg agctgtgctg cgactcgtgg cgtaatcatg gtcatagctg
      541 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      601 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      661 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      721 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gtcgcttacc
     1081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1681 tggtctgaca gttagaaaaa ctcatcgagc atcaagtgaa actgcaattt attcatatca
     1741 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1801 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1861 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1921 tgagtgacga ctgaatccgg tgagaatggc aagagtttat gcatttcttt ccagacttgt
     1981 tcaacaggcc agccattacg ctcgtcatca aaatcactcg caccaaccaa accgttattc
     2041 attcgtgatt gcgcctgagc gaaacgaaat acgcgatcgc cgttaaaagg acaattacaa
     2101 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2161 gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt
     2221 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2281 gtcagccagt ttagcctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2341 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2401 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2461 tttaatcgcg gcttcaagca agacgtttcc cgttgaatat ggctcatttt agcttcctta
     2521 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2581 tgaaagttgg aacctcttac gtgccgatca cacatttccc cgaaaagtgc cacctgacgt
     2641 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2701 ttgtc
//